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KMID : 0378019690120100045
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New Medical Journal
1969 Volume.12 No. 10 p.45 ~ p.51
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Radioautographic Studies on Renewal of the Hepatic Cell of Normal Adult Male Mice using ^(3)H-thymidine
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서병호/Suh, Byung Ho
강준원/정진웅/최월봉/Kang, Jun Won/Chung, Jin Woong/Choi, wol Bong
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Abstract
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Normal male 6 week old mice(D. D. 0. /Lee) weighing about 20 grams were used for this study. 100 mice were equally divided into 20 groups(five to every group) and each animal was intravenously given a single dose of 0.8 pCi per gm body weight of 3H-thymidine. First in the diurnal variation of DNA synthesis the values were found highest in the morning(6 a. m.) group among the 4 differentiated ones, the remaining 16 groups were each injected intravenously the above isotope at 6 a. m. simultaneously, 1, 2, 3, 4, 5, 6, 7, 8, 9, 10, 15, 30, 48, 96, 192, 384 hours after which some specimens of liver were excised. The authors present here the following results showing some data on the problems concerning cell renewal investigated by autoradiographic technique.
1. The values of DNA synthesis of the hepatic cells in the 4 per diem differentiated groups are highest in the 6 a. m. group (3.33), rank second in the midnight(1.80) and evening(1.20) groups and are lowest in the noon group(0.93). And in general, the values of DNA synthesis of the hepatic cells in the adult male mice are rather low.
2. The renewal or generation time of the hepatic cells in the adult male mice is put at about 240 hours. 3. The renewed-hepatic cells become degenerate at least within 384 hours.
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